Real-time eDNA analysis
The Therkildsen Lab does real-time sequencing on a tiny, portable Nanopore MinION sequencer! We are using the sequencer for an eDNA project as part of our class NTRES 3400 (Molecular Tools for Ecology, Conservation, and Natural Resource Management) taught by Nina. Eileen Randall and Kurt Jika from Cliff Kraft's Lab helped us with field sampling at two of our sampling sites so we could compare the fish species composition inferred with metabarcoding and electrofishing.
Later in the summer, we will use the MinION sequencer to generate long reads for our silverside genome assembly.